Faststart sybr green master is a ready to use hot start reaction mix without rox for quantitative polymerase chain reaction qpcr and reverse transcription rt qpcr on real time pcr systems other than the lightcycler instruments. Fast sybr green master mix was formulated to minimize primer dimerization and non specific amplification to optimize the efficiency and accuracy of the reaction without compromising sensitivity. As the pcr reaction proceeds at each round of amplification sybr green dye binds to dsdna as it polymerizes resulting in an increase in the level of fluorescence at the end of each extension step.
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